---
_id: '2737383'
abstract:
- lang: eng
text: Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far
in legume nodules, despite the fact that reactive oxygen species are produced
at different steps of the symbiosis. The function of two Gpxs that are highly
expressed in nodules of the model legume Lotus japonicus was examined. Gene expression
analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ
mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein
(GFP) fusions were used to characterize the enzymes and to localize each transcript
and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent
phospholipid hydroperoxidases and are differentially regulated in response to
nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or
in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification
of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this
modification results in enzyme inhibition. The enzymes are highly expressed in
the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular
bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol
and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes
protect against oxidative stress, salt stress, and membrane damage. It is concluded
that both LjGpxs perform major antioxidative functions in nodules, preventing
lipid peroxidation and other oxidative processes at different subcellular sites
of vascular and infected cells. The enzymes are probably involved in hormone and
NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine
essential for catalytic function.
article_type: original
author:
- first_name: Manuel A.
full_name: Matamoros, Manuel A.
last_name: Matamoros
- first_name: Ana
full_name: Saiz, Ana
last_name: Saiz
- first_name: Maria
full_name: Peñuelas, Maria
last_name: Peñuelas
- first_name: Pilar
full_name: Bustos-Sanmamed, Pilar
last_name: Bustos-Sanmamed
- first_name: Jose M.
full_name: Mulet, Jose M.
last_name: Mulet
- first_name: Maria V.
full_name: Barja, Maria V.
last_name: Barja
- first_name: Nicolas
full_name: Rouhier, Nicolas
last_name: Rouhier
- first_name: Marten
full_name: Moore, Marten
last_name: Moore
- first_name: Euan K.
full_name: James, Euan K.
last_name: James
- first_name: Karl-Josef
full_name: Dietz, Karl-Josef
last_name: Dietz
- first_name: Manuel
full_name: Becana, Manuel
last_name: Becana
citation:
ama: Matamoros MA, Saiz A, Peñuelas M, et al. Function of glutathione peroxidases
in legume root nodules. Journal of Experimental Botany. 2015;66(10):2979–2990.
apa: Matamoros, M. A., Saiz, A., Peñuelas, M., Bustos-Sanmamed, P., Mulet, J. M.,
Barja, M. V., Rouhier, N., et al. (2015). Function of glutathione peroxidases
in legume root nodules. Journal of Experimental Botany, 66(10),
2979–2990.
apa_indent:
Matamoros,
M. A., Saiz, A., Peñuelas, M., Bustos-Sanmamed, P., Mulet, J. M., Barja, M. V.,
Rouhier, N., et al. (2015). Function of glutathione peroxidases in legume root
nodules. Journal of Experimental Botany, 66(10), 2979–2990.
aps: ' M. A. Matamoros, et al., Function of glutathione peroxidases in legume root
nodules, Journal of Experimental Botany 66, 2979–2990 (2015).'
bio1: 'Matamoros MA, Saiz A, Peñuelas M, Bustos-Sanmamed P, Mulet JM, Barja MV,
Rouhier N, Moore M, James EK, Dietz K-J, Becana M (2015)
Function of glutathione
peroxidases in legume root nodules.
Journal of Experimental Botany 66(10):
2979–2990.'
default: 'Matamoros MA, Saiz A, Peñuelas M, Bustos-Sanmamed P, Mulet JM, Barja MV,
Rouhier N, Moore M, James EK, Dietz K-J, Becana M (2015)
Journal of Experimental
Botany 66(10): 2979–2990.'
dgps: Matamoros,
M.A., Saiz, A., Peñuelas, M., Bustos-Sanmamed, P., Mulet, J.M., Barja, M.V., Rouhier,
N., Moore, M., James, E.K., Dietz, K.-J. & Becana, M. (2015). Function of
glutathione peroxidases in legume root nodules. Journal of Experimental Botany,
66(10), 2979–2990. doi:10.1093/jxb/erv066.
ewi: 'Matamoros, M.A., Saiz, A., Peñuelas, M., Bustos-Sanmamed, P., Mulet, J.M.,
Barja, M.V., Rouhier, N., Moore, M., James, E.K., Dietz, K.-J., & Becana,
M.: Function of glutathione peroxidases in legume root nodules Journal of
Experimental Botany,66:(10): 2979–2990.'
frontShortTitle: 'Function of glutathione peroxidases in legume root nodules
Matamoros MA, Saiz A, Peñuelas M, Bustos-Sanmamed P, Mulet JM, Barja MV, Rouhier
N, Moore M, James EK, Dietz K-J, Becana M (2015)
Journal of Experimental
Botany 66(10): 2979–2990.'
frontiers: Matamoros, M. A., Saiz, A., Peñuelas, M., Bustos-Sanmamed, P., Mulet,
J. M., Barja, M. V., Rouhier, N., Moore, M., James, E. K., Dietz, K. - J., et
al. (2015). Function of glutathione peroxidases in legume root nodules. Journal
of Experimental Botany 66, 2979–2990.
harvard1: Matamoros, M.A., et al., 2015. Function of glutathione peroxidases in
legume root nodules. Journal of Experimental Botany, 66(10), 2979–2990.
ieee: ' M.A. Matamoros, et al., “Function of glutathione peroxidases in legume root
nodules”, Journal of Experimental Botany, vol. 66, 2015, pp. 2979–2990.'
lncs: ' Matamoros, M.A., Saiz, A., Peñuelas, M., Bustos-Sanmamed, P., Mulet, J.M.,
Barja, M.V., Rouhier, N., Moore, M., James, E.K., Dietz, K.-J., Becana, M.: Function
of glutathione peroxidases in legume root nodules. Journal of Experimental Botany.
66, 2979–2990 (2015).'
mla: 'Matamoros, Manuel A., Saiz, Ana, Peñuelas, Maria, Bustos-Sanmamed, Pilar,
Mulet, Jose M., Barja, Maria V., Rouhier, Nicolas, Moore, Marten, James, Euan
K., Dietz, Karl-Josef, and Becana, Manuel. “Function of glutathione peroxidases
in legume root nodules”. Journal of Experimental Botany 66.10 (2015):
2979–2990.'
pub: Matamoros, M.A., Saiz, A., Peñuelas, M., Bustos-Sanmamed, P., Mulet, J.M.,
Barja, M.V., et al. 2015. “Function of glutathione peroxidases in legume root
nodules”. Journal of Experimental Botany 66(10), 2979–2990.
sfb882: 'Matamoros, Manuel A., Saiz, Ana, Peñuelas, Maria, Bustos-Sanmamed, Pilar,
Mulet, Jose M., Barja, Maria V., Rouhier, Nicolas, Moore, Marten, James, Euan
K., Dietz, Karl-Josef, & Becana, Manuel (2015): Function of glutathione peroxidases
in legume root nodules. Journal of Experimental Botany, 66(10),
2979–2990'
wels: 'Matamoros, M. A.; Saiz, A.; Peñuelas, M.; Bustos-Sanmamed, P.; Mulet, J.
M.; Barja, M. V.; Rouhier, N.; Moore, M.; James, E. K.; Dietz, K. - J.; Becana,
M. (2015): Function of glutathione peroxidases in legume root nodules Journal
of Experimental Botany,66:(10): 2979–2990.'
date_created: 2015-05-22T08:35:10Z
date_updated: 2021-06-10T12:54:29Z
department:
- _id: '2685177'
doi: 10.1093/jxb/erv066
external_id:
isi:
- '000356223900015'
pmid:
- '25740929'
intvolume: ' 66'
isi: 1
issue: '10'
language:
- iso: eng
page: 2979-2990
pmid: 1
publication: Journal of Experimental Botany
publication_identifier:
issn:
- 1460-2431
publication_status: published
quality_controlled: '1'
status: public
title: Function of glutathione peroxidases in legume root nodules
type: journal_article
user_id: 1234
volume: 66
year: '2015'
...